For PAX5 (E inset). PAX5 (E) and CD21 (F) show the moth eaten appearance of nodules made by the atypical T-cell clusters constructive for CDAm J Surg Pathol. Author manuscript; obtainable in PMC 2014 June 01.Nicolae et al.Web page(G) and CD4 (H). Hodgkin-Reed Sternberg-like cells are rosetted by CD3 (G) and CD4 (H inset) positive T-cells. (A, B, C, E, F ?case 4; D, G, H – case 5).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptAm J Surg Pathol. Author manuscript; obtainable in PMC 2014 June 01.Nicolae et al.PageNIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 4.Attributes of neoplastic T-cells in cases of PTCL, follicular variant, with EBV-negative HRSlike cells. A. CD3 immunostain highlights a sizable aggregate of atypical T-cells showing variation in nuclear size and shape.130473-38-0 custom synthesis B. Quite a few nodules of atypical T-cells are highlighted by PD-1 immunostain. Two nodules on right contain HRS-like cells. C. Many from the atypical Tcells are optimistic for CD10, with some rosetting HRS-like cells. D. Similarly, the atypical Tcells are optimistic for Bcl-6, although HRS-like cell is adverse. The huge size from the T-cell aggregates, as well as the atypical immunophenotype (CD10-posiitve, Bcl-6-positive, strong and uniform PD-1) are clues against the diagnosis of lymphocyte-rich CHL. (A, B, C ?case 4; D ?case five).NIH-PA Author ManuscriptAm J Surg Pathol. Author manuscript; obtainable in PMC 2014 June 01.Nicolae et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 5.PCR research of PTCL-NOS, follicular variant, containing HRS-like cells (Case 5). TRG PCR identifies two identical peaks, constant with a clonal rearrangement in all 3 specimens tested: soft tissue 2011 (A), inguinal lymph nodes 2006 (B) and 2004 (C). This confirms that the identical disease procedure is present in various sites and at different time points at molecular level. Color code of interrogated joining segments of TCR locus: black ?J1/2; green ?JP1/2 and blue ?JP.Am J Surg Pathol. Author manuscript; out there in PMC 2014 June 01.5-Amino-6-methylnicotinonitrile web Nicolae et al.PMID:23546012 PageTableAntibodies applied within the immunophenotypic analysesNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptAntigen CD3 CD4 CD8 CD10 CD15 CD20 CD21 CD30 CD79a PAX5 Oct-2 Bcl-6 MUM1 PD-1 (CD279) IgD Kappa Lambda LMPClone Polyclonal 1F6 C8/144B 56CF Leu-M1 L26 1F8 1G12 JCB117 24 CD-20 PG-B6p MUM1p NAT Polyclonal Polyclonal Polyclonal C-S 1?Dilution 1:one hundred 1:40 1:50 1:20 1:20 1:200 1:30 1:80 1:100 1:25 1:4000 1:20 1:one hundred 1:50 1:1000 1:25000 1:10000 1:Source Dako Novocastra Dako Novocastra Becton Dickinson Dako Dako Novocastra Dako BD Transl Labs Santa Cruz Dako Dako ABCAM Dako Dako Dako DakoAm J Surg Pathol. Author manuscript; obtainable in PMC 2014 June 01.TableClinical parameters and molecular status of all PTCL with HRS-like cellsNo instances 32 65 (31?six) 14/18 Age Median (range) Gender M/F Location TRG@* 25 – c two – pc 2 – suspicious 1 ?inconclusive 15 – c 1 – computer 1 – suspicious 2-c 7 – pc 1 – suspicious 4-c 19 – pc IG@*Nicolae et al.Lymphoma typeAITL#LN: 8 axilla, eight cervical, 11 inguinal, 3 supraclavicular, 2 NOS LN: 11 cervical, 2 inguinal, 1 periparotid, 1 supraclavicular, 1 axilla, 1 bone marrow; 1 NOS 1 tongueLN: two cervical, 1 inguinal, axilla and skin/soft tissue LN: inguinal and axilla LN: two cervical 2-c 2- pc 1-c 1 – pc 3-c 2- pcPTCL-NOS62 (35?eight)12/PTCL-NOS, Follicular variant57 (52?7)2/PTCL-NOS, T-zone 2 57 59.five (53?6) 2/1/ATLLTotalHRS ?Hodgkin/Reed-Sternberg; AITL ?angioimmu.
